Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins
- 16 January 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 296 (3) , 300-304
- https://doi.org/10.1016/0014-5793(92)80309-5
Abstract
A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstratedKeywords
This publication has 11 references indexed in Scilit:
- Western blots from sodium dodecyl sulfate-polyacrylamide gels stained by metal saltsAnalytical Biochemistry, 1989
- Sodium dodecyl sulfate-gel electrophoresis: Staining of polypeptides using heavy metal saltsAnalytical Biochemistry, 1988
- Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gelsAnalytical Biochemistry, 1987
- Isolation and elution of proteins from sodium dodecyl sulfate-polyacrylamide gels with an intermediate agarose-containing layer in the acrylamide gelAnalytical Biochemistry, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnalytical Biochemistry, 1980
- Rapid visuallzation of protein-dodecyl sulfate complexes in polyacrylamide gelsAnalytical Biochemistry, 1976
- A rapid, sensitive, and specific method for the determination of protein in dilute solutionAnalytical Biochemistry, 1973
- A new sensitive, rapid fluorescence technique for the determination of proteins in gel electrophoresis and in solutionAnalytical Biochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970