In vitro cellular response to polypyrrole-coated woven polyester fabrics: Potential benefits of electrical conductivity

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Abstract
Electrically conducting polypyrrole‐treated films have recently been shown to influence the morphology and function of mammalian cells in vitro. This type of polymer represents a possible alternative biomaterial for use in vascular implantation. The present study compared the in vitro biocompatibility of five different polyester woven fabrics having increasing levels of electrical conductivity ranging from 4.5 × 104 to 123 Ω/square with that of low density polyethylene and polydimethylsiloxane primary reference materials. Biocompatibility was measured in terms of four different types of in vitro cellular response, including (a) an indirect and (b) a direct control organotypic culture assay using endothelial cells, (c) a polymorphonuclear (PMN) cell activation study using flow‐cytometric measurements of CD11/CD18 integrin molecule expression, and (d) a semiquantification of interleukin (IL)‐6 mRNA expression on monocytes/macrophages using reverse‐transcriptase polymerase chain reaction. The organotypic culture study revealed that the fabrics with high levels of conductivity exhibited lower cell migration, proliferation, and viability. The PMN activation study of blood from 10 healthy adult donors demonstrated that the two most conductive fabrics were able to identify the more reactive donors. The levels of IL‐6 mRNA expression by monocytes/macrophages decreased as the conductivity level of the fabrics increased. The results of the present study therefore indicate that high levels of conductivity (3 < 104 Ω/square) which is associated with an optimum in vitro cellular response. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 519–526, 1998.

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