Formation of functional inter‐species hybrid photosynthetic complexes in Rhodobacter capsulatus
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2) , 247-252
- https://doi.org/10.1016/0014-5793(89)80969-x
Abstract
A Rhodobacter capsulatus mutant strain deficient in all pigment-binding peptides and hence incapable of photosynthetic growth was genetically complemented with a plasmid-borne copy of the Rhodobacter sphaeroides puf operon. Hybrid reaction centers composed of R. sphaeroides L and M and R. capsulatus H subunits assembled in vivo, and host cells were photosynthetically competent. Light-harvesting complex B875, also encoded by the R. sphaeroides puf operon, was present along with the hybrid reaction center. These cells emitted fluorescence, however, indicating an impairment in energy transduction.Keywords
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