Difference in Saponin Sensitivity between Myotubes and Mononucleated Cells from Chick Breast Muscle

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Abstract
Cultured muscle cells treated successively with saponin at varied concentrations released protein initially at 2 .times. 10-5 g/ml saponin and then at 6 .times. 10-5 g/ml saponin. The 1st release was accompanied with creatine kinase activity, whereas the 2nd release was not. Morphological observations indicated that the 1st protein leakage was derived mainly from myotubes and the 2nd release mainly from mononucleated cells.