FLUORESCEIN-LABELED ESTRADIOL - PROBE FOR ANTI-ESTRADIOL ANTIBODY
- 1 January 1977
- journal article
- research article
- Vol. 18 (1) , 147-156
Abstract
A fluorescent estradiol derivative binds strongly to anti-estradiol antibody. [Bovine serum albumin was used to induce the antibody.] The binding, measured by fluorescence polarization, is inhibited by estradiol and by diethylstilbestrol. Tentatively characterized as N-(estradiol-6-iminooxyacetyl) fluorescein amine, the derivative was prepared from estradiol-6-iminooxyacetic acid, dicyclohexylcarbodiimide, and fluorescein amine, and isolated by TLC. It has a fluorescence emission similar to that of fluorescein and an absorption spectrum consistent with a fluorescein: estradiol molar ratio of 1:1.This publication has 3 references indexed in Scilit:
- A fluorescein-labeled derivative of estradiol with binding affinity towards cellular receptorsBiochemical and Biophysical Research Communications, 1977
- FURTHER OBSERVATIONS ON THE KOBER COLOUR AND ITTRICH FLUORESCENCE REACTIONS IN THE MEASUREMENT OF OESTRIOL, OESTRONE AND OESTRADIOLJournal of Endocrinology, 1968
- The synthesis of fluorescent penicilloyl haptens and their use in investigating ‘penicillin’ antibodies by fluorescence polarizationImmunochemistry, 1967