Biophysical characterization of OprB, a glucose-inducible porin ofPseudomonas aeruginosa
- 1 October 1993
- journal article
- research article
- Published by Springer Nature in Journal of Bioenergetics and Biomembranes
- Vol. 25 (5) , 547-556
- https://doi.org/10.1007/bf01108411
Abstract
OprB, a glucose-inducible porin ofP. aeruginosa, was characterized by black lipid bilayer analysis and circular dichroism spectroscopy. Black lipid bilayer analysis of OprB revealed a single-channel conductance of 25 pS, the presence of a glucose binding site with aK s for glucose of 380 ± 40 mM, and the formation of channels with a strong selection for anions. Analysis ofP. aeruginosa OprB circular dichroism spectra revealed a high β sheet content (40%) which is within the range of that determined for other porins. Values obtained from black lipid bilayer analysis were compared to those previously obtained for OprB ofP. putida [Saravolacet al. (1991).J. Bacteriol. 173, 4970–4976] and indicated extensive similarities in the single-channel conductance and glucose-binding properties of these two porins. Immunological and amino terminal sequence analysis revealed a high degree of homology. Of the first 14 amino terminal residues, 12 were identical. A major difference between the two porins was found in their ion selectivity. WhereasP. aeruginosa OprB is anion selective,P. putida OprB and other carbohydrate selective porins are known to be cation selective.Keywords
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