Metabolism of the Fungicide Denmert®(S-n-Butyl S′-p-tert-Butylbenzyl N-3-Pyridyldithiocarbonimidate, S-1358) and Denmert Sulfoxides in Liver Enzyme Systems

Abstract
On incubation with rat liver microsomes plus NADPH, Denmert (S-n-butyl S′-p-tert-butylbenzyl N-3-pyridyldithiocarbonimidate) underwent at least two different types of oxidation; hydroxylation and sulfoxidation. Hydroxylation of Denmert at the tert-butyl group was one of the major metabolic attacks in mammalian metabolism. Sulfoxidation of Denmert gave two isomers of Denmert sulfoxides which were intermediates in the metabolism and readily transformed into 2-(3′-pyridylimmo)-4-carboxylthiazolidine (HM) in the presence of l-cysteine without enzymatic mediation. This type of conjugation with cysteine appears to be a new class of metabolic reactions in mammals. Denmert S-oxide showed increased fungicidal activity when assayed in liquid cultures, but not on plant leaves.

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