A method for investigating the role of calcium in the shape change, aggregation and serotonin release of rat platelets.

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Abstract
Effects of the platelet secretagogues thrombin and concanavalin (Con) A on shape change and release were studied under conditions where external Ca2+ and ADP were controlled. In the absence of detectable aggregation both Con A- and thrombin-induced release of serotonin are markedly dependent on extracellular Ca. Con A, unlike thrombin, does not produce aggregation in Ca-supplemented medium. In combination with added creatine phosphate and creatine phosphokinase, Con A will be of value in the analysis of Ca-dependent release in platelets.