Distinct Modes of Signal Recognition Particle Interaction with the Ribosome
- 23 August 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 297 (5585) , 1345-1348
- https://doi.org/10.1126/science.1072366
Abstract
Signal recognition particle (SRP), together with its receptor (SR), mediates the targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Using protein cross-linking, we detected distinct modes in the binding of SRP to the ribosome. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts SR, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.Keywords
This publication has 29 references indexed in Scilit:
- The Structural Basis of Ribosome Activity in Peptide Bond SynthesisScience, 2000
- The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 Å ResolutionScience, 2000
- Role of Sec61α in the Regulated Transfer of the Ribosome–Nascent Chain Complex from the Signal Recognition Particle to the Translocation ChannelCell, 2000
- Regulation by the ribosome of the GTPase of the signal-recognition particle during protein targetingNature, 1996
- A posttargeting signal sequence recognition event in the endoplasmic reticulum membraneCell, 1995
- SRP samples nascent chains for the presenceof signal sequences by interacting with ribosomes at a discrete step during translation elongationCell, 1995
- Signal-sequence recognition by an Escherichia coli ribonucleoprotein complexNature, 1992
- The signal sequence of nascent preprolactin interacts with the 54K polypeptide of the signal recognition particleNature, 1986
- Elongation arrest is not a prerequisite for secretory protein translocation across the microsomal membrane.The Journal of cell biology, 1985
- Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle.The Journal of cell biology, 1982