Allagochrome. I. Properties, Purification, & Assay Procedures

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Abstract
Allagochrome is a blue-green, water soluble pigment which exhibits reversible changes in color in response to reduction (to yellow) and acidification (to red). It is extracted from leaves by grinding in alkaline buffer. The resulting brei is centrifuged to remove solids and further purification is by continuous flow electrophoresis and chromatography on sephadex. The assay procedure for allagochrome is based on differences in optical density between oxidized and reduced aliquots of crude extracts. Known sources having high concentrations of the pigment are listed. Comparative studies of purified allagochrome prepared from Helianthus, Chrysanthemum, and Ligustrum included determinations of Cu and P content, changes on absorption spectra with pH, and fluorescence characteristics. Present estimates of molecular size and likely composition, nature of the chromophore and fluorophore and possible relationships with other pigment systems are discussed.

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