Observations on the binding of haemoglobin and haematin by serum proteins in the rabbit, rat and guinea pig

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Abstract
The complexes formed by the addition of haemoglobin and haematin to sera obtained from the rabbit, rat and guinea pig have been investigated by paper electrophoresis. In the rabbit, a haemoglobin-haptoglobin complex migrates with the [alpha]2-globulins and a haematin-protein complex migrates with albumin and with the [alpha]2-globulins. In the rat, a haemoglobin-haptoglobin complex migrates with the [alpha]2-globulins and a haematin-protein complex migrates with albumin and with the [beta]1-globulins. In the guinea pig, haemoglobin-haptoglobin complexes migrate with albumin and [alpha]3-globulin; in some sera, a complex probably also migrates with the [beta]-globulins. Haematin-protein complexes migrate with albumin, [alpha]3-globulin and [beta]-globulin. In the rabbit, the haemoglobin-haptoglobin complex was removed from the plasma with a half-time of 40 min.