A Comparison of Fertility of Bovine Spermatozoa Stored at 5° C. and −79° C

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Abstract
The fertility of frozen semen was compared with the fertility of unfrozen semen on a split-sample basis. Semen from bulls of the Holstein, Guernsey, Jersey, Brown Swiss, and Angus breeds was frozen and stored in glycerolated yolk-citrate extender for periods of time ranging from 1 day to 4 months. The average 60- to 90_day nonreturn rate for a total of 2163 services to frozen semen was 59% and for 6661 services to unfrozen semen stored at 5[degree]C the nonreturn rate was 71%. In one trial comparing fertility of unfrozen and frozen semen on a within and among bull basis, the mean 60-to 90-day nonreturn rate for 539 services to unfrozen semen was 68%, and for 439 services to frozen semen, 47%. The analysis of variance revealed a highly significant difference between unfrozen semen and frozen semen (P< 0.01), a significant difference among bulls (P< 0.05), and no significant difference within bulls (among ejaculates from the same bull). In another trial, polyethylene bulbs were compared with glass ampules as containers for frozen semen. The 60- to 90-day nonreturn rate was 62.3% for 313 services to samples frozen in polyethylene bulbs and 60.7% for 298 services to samples frozen in glass ampules. The difference was not significant at the 5% level of probability.

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