The Function of the Carbohydrate Units of Three Fungal Enzymes in Their Resistance to Dehydration
Open Access
- 1 November 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 54 (5) , 717-721
- https://doi.org/10.1104/pp.54.5.717
Abstract
Glucose oxidase (from Aspergillus niger), glucoamylase (from Rhizopus spp.), and cellulase (from Aspergillus niger) of fungal origin are all glycosylated proteins. Dehydration of the three enzymes to a range of water potentials did not affect their activity. However, when more than 10% of the carbohydrate associated with the molecules was removed by periodate oxidation, the enzymes were highly susceptible to dehydration when compared with oxidized controls. Polyvinyl pyrrolidone and Dextran T500 protected the three enzymes in their oxidized state against the effects of dehydration.Keywords
This publication has 18 references indexed in Scilit:
- The influence of dehydration on catalase stability. A comparison with freezing effectsCryobiology, 1974
- Mechanism of cryoinjury in biological systemsCryobiology, 1972
- Periodate oxidation of carbohydrate moiety of stem bromelain without much alteration in enzymic activityBiochemistry, 1971
- Glycoenzymes: A note on the role for the carbohydrate moietiesBiochemical and Biophysical Research Communications, 1970
- GlycoproteinsAnnual Review of Biochemistry, 1970
- Extracellular enzyme system utilized by the rot fungus Stereum sanguinolentum for the breakdown of celluloseArchives of Biochemistry and Biophysics, 1968
- On the biological role of glycoproteinsJournal of Theoretical Biology, 1966
- A glycoprotein structure for glucose oxidase from Aspergillus nigerArchives of Biochemistry and Biophysics, 1965
- The glycoprotein nature of some fungal carbohydrasesArchives of Biochemistry and Biophysics, 1963
- Protein Hydration and BehaviorScience, 1958