COMPLETE ENZYMIC DIGESTION OF ACIDIC PROTEINS
- 1 July 1976
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 8 (4) , 379-383
- https://doi.org/10.1111/j.1399-3011.1976.tb02517.x
Abstract
Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of “unusual” amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins. The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino-peptidase plus prolidase followed by carboxypeptidase C, is described. Recoveries of amino acids were in excellent agreement with the expected content from the known sequences.Keywords
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