An Affinity‐Modulating Site on Neuronal Monoamine Transport Proteins

Abstract
The dissociation rates of [3H]nisoxetine, [3H]GBR 12935 and [3H]citalopram from, respectively, the rat brain noradrenaline, dopamine and 5‐HT transporters were found to be markedly affected by several drugs. Sertraline strongly attenuated the rate of dissociation of [3H]nisoxetine from the noradrenaline transporter, while citalopram strongly attenuated that of [3H]citalopram from the 5‐HT transporter. The effects of both drugs were stereospecific. Less potent affinity‐modulating drugs were identified with regards to [3H]GBR 12935 dissociation from the dopamine transporter. All three neuronal monoamine transporters may thus have specific affinity‐modulating sites which change the function of the transporters with possible implications for the reuptake of monoamines released during synaptic activity.

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