The first observation of O2 − generation at real time in vivo from non-Kupffer sinusoidal cells in perfused rat liver during acute ethanol intoxication

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Abstract
Infusion of ethanol or phorbol myristate acetate (PMA) into the perfused rat liver immediately produces O2 − which was detected directly by infusion of a Cypridina luciferin analogue, MCLA as a chemiluminescence reagent. The MCLA photon emission was inhibitable by SOD. Generation of O2 − in the liver was further verified by nitroblue tetrazolium, formazan precipitate formation. Ethanol‐induced O2 − generation was unaffected by gadolinium chloride (GdCl3), an inhibitor of kupffer cells, while PMA induced O2 − generation was completely abolished by GdCl3, Since PMA is a known stimulator of phagocytic cells including Kupffer cells, the results indicate, for the first time that ethanol stimulates a non‐Kupffer cell population, probably liver sinusoid endothelial cell to produce O2 −