The effect of fatty acid chain length and unsaturation on the chromatographic behavior of triglycerides on latroscan chromarods

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Abstract
The chromatographic behavior of lipid classes on Chromarods with common developing solvents used for the Iatroscan system was influenced by the esterified fatty acids present in the lipid class. The Rf value of the lipid class increased with increasing chain length and increasing unsaturation of the fatty acids; the increase in Rf value per methylene carbon was about twice that per double bond. The effect was evident in both triglycerides and phosphatidylcholines. This partial separation of molecular species within a lipid class caused widening of the lipid class band on the Chromarods and can result in poorer resolution, particularly in biological samples.