Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. ?Centennial?
- 1 October 1992
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 11 (11) , 581-585
- https://doi.org/10.1007/bf00233097
Abstract
Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar ‘Centennial’ on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. ‘Centennial’ expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.Keywords
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