Abstract
Freshly prepared testicular homogenate of sexually mature catfish (Clarias macrocephalus) stored undiluted or diluted with distilled water (1:1 by volume) at 0-1 C (32-33.8 F) retained its initial fertility up to 20 hours. After 24 hours of storage at 0-1 C (32-33.8 F), its fertility decreased significantly such that at 32 hours of storage, it was no longer fertile. The ability of the testicular homogenate to retain its initial fertility for 20 hours allows this species to be efficiently cultured.