Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters
- 1 October 1992
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 58 (10) , 3419-3422
- https://doi.org/10.1128/aem.58.10.3419-3422.1992
Abstract
A 26-mer oligonucleotide specific to Vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. This oligonucleotide probe specifically reacted with DNA from 89 of 95 V. parahaemolyticus isolates but not with DNA from other vibrios or other enteric and nonenteric organisms (n = 48). The probe hybridized with Southern blots of 0.5-kb HindIII-restricted chromosomal DNA fragments from all but five V. parahaemolyticus test isolates. The probe could be used to directly identify V. parahaemolyticus in artificially contaminated food without an isolation step.Keywords
This publication has 7 references indexed in Scilit:
- Evaluation of hybridization characteristics of a cloned pRF106 probe for Listeria monocytogenes detection and development of a nonisotopic colony hybridization assayApplied and Environmental Microbiology, 1991
- Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenesApplied and Environmental Microbiology, 1988
- Synthetic oligodeoxyribonucleotide probes to detect Kanagawa phenomenon-positive Vibrio parahaemolyticusJournal of Clinical Microbiology, 1986
- Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticusJournal of Bacteriology, 1985
- Rapid transfer of DNA from agarose gels to nylon membranesNucleic Acids Research, 1985
- DNA-DNA hybridization assay for detection of Salmonella spp. in foodsApplied and Environmental Microbiology, 1983
- Vibrio Parahaemolyticus and Related Halophilic VibriosCRC Critical Reviews in Microbiology, 1982