Detection of TDH-producing Vibrio parahaemolyticus O3: K6 from Naturally Contaminated Shellfish Using an Immunomagnetic Separation Method and Chromogenic Agar Medium
- 1 January 2001
- journal article
- Published by The Japanese Association for Infectious Diseases in Kansenshogaku Zasshi
- Vol. 75 (11) , 955-960
- https://doi.org/10.11150/kansenshogakuzasshi1970.75.955
Abstract
We attempted to isolate TDH-producing Vibrio parahaemolyticus O3:K6 from shellfish. Asari samples were incubated with TSB supplemented with 2% (w/v) NaCl for 6 h, and then the 6-h cultures were incubated with salt polymyxin broth for 18 h. After the two-step enrichment, a 1 ml portion of the culture was treated with magnetic beads coated with K6 antibody for immunoconcentration of V. parahaemolyticus O3:K6. The immunoconcentrated and untreated cultures were plated onto a chromogenic agar and TCBS agar media for isolation of V. parahaemolyticus. TDH-producing V. parahaemolyticus O3:K6 was isolated from 3 out of 66 lots (4.5%) of naturally contaminated Asari. Six of 4,265 colonies suspected as V. parahaemolyticus (0.14%) were TDH-producing V. parahaemolyticus O3:K6.Keywords
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