Unequivalence between Hemoglobin Subunits

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Abstract
The effects of inositol hexakisphosphate on the visible absorption spectrum of liganded valency hybrids α2COβ2+H2O, α2+H20β2CO and α2COβ2+CN, α2+CNβ2co (where αCO, α+H2O, α+CN and βCO, β+H2O, β+CN represent the α and β chain of hemoglobin, respectively, in their carbon monoxide, aquomet and eyanomet forms) have been examined in an attempt to determine if their α and β chains are equally sensitive to the action of the anion.The difference spectra induced by inositol hexakisphosphate, are different for the two chains. The β+H2O subunit contributes chiefly to the absorbance changes, rather than α+H2O. In contrast, it is the αCO chain which is more sensitive to inositol hexakisphosphate than βCO. The question ofwhether such a selective response would also occur in aquomethemoglobin and carboxyhemoglobin is discussed. Criteria of changes in the quaternary structure of hybrids has been studied by measuring the effects of inositol hexakisphosphate on their ultraviolet absorption spectrum.

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