Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae

22 August 2006

journal article
Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences

Vol. 103 (34) , 12819-12824
https://doi.org/10.1073/pnas.0605778103

Abstract

We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae . A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V ( CEN5-CAN1 ), the rate of mitotic cross-overs was 4 × 10 ⁻⁵ per division, a rate ≈25,000-fold lower than the meiotic rate of cross-overs. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.

Keywords

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