Studies on Cyclodextrin Glycosyltransferase. IV. Enzymatic Synthesis of 3-O-α-D-Glucopyranosyl-L-sorbose and 4.O-α-D-Glucopyranosyl-D-xylose Using Cyclodextrin Glycosyltransferase
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 79 (3) , 641-648
- https://doi.org/10.1093/oxfordjournals.jbchem.a131108
Abstract
The acceptor specificity of the transglycosylation reaction of cyclodextrin glycosyl-transferase[EC 2.4.1.19] was investigated using various sugars and sugar alcohols. L-Sorbose, D-xylose, and D-galactose, which contain configurational or structural changes relative to the D-glucopyranose unit at positions other than position 1, were also shown to be efficient acceptors in the transglycosylation reaction of this enzyme. It was shown by chemical and enzymatic methods that this enzyme could transfer glycosyl residues only to the C3hydroxyl group of L-sorbose and C4-hydroxyl group of D-xylose, producing oligosaccharides terminated by 3-O-α-D-gIucopyranosyl-L-sorbose and 4-O-α-D-glucopyranosyl-D-xylose at the reducing ends, respectively.This publication has 1 reference indexed in Scilit:
- The Conversion of Starch to Crystalline Dextrins by the Action of a New Type of Amylase Separated from Cultures of Aerobacillus maceransJournal of the American Chemical Society, 1939