Homologous recombination promoted by reverse transcriptase during copying of two distinct RNA templates.
- 18 July 1995
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 92 (15) , 6971-6975
- https://doi.org/10.1073/pnas.92.15.6971
Abstract
Retroviruses are known to mutate at high rates. An important source of genetic variability is recombination taking place during reverse transcription of internal regions of the two genomic RNAs. We have designed an in vitro model system, involving genetic markers carried on two RNA templates, to allow a search for individual recombination events and to score their frequency of occurrence. We show that Moloney murine leukemia virus reverse transcriptase alone promotes homologous recombination efficiently. While RNA concentration has little effect on recombination frequency, there is a clear correlation between the amount of reverse transcriptase used in the assay and the extent of recombination observed. Under conditions mimicking the in vivo situation, a rate compatible with ex vivo estimates has been obtained.Keywords
This publication has 11 references indexed in Scilit:
- Recombinant HIV-1 Nucleocapsid Protein Accelerates HIV-1 Reverse Transcriptase Catalyzed DNA Strand Transfer Reactions and Modulates RNase H ActivityBiochemistry, 1994
- Fidelity of in vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse TranscriptaseBiochemistry, 1994
- Mechanism of DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse TranscriptaseScience, 1992
- Broad spectrum of in vivo forward mutations, hypermutations, and mutational hotspots in a retroviral shuttle vector after a single replication cycle: deletions and deletions with insertions.Proceedings of the National Academy of Sciences, 1990
- Broad spectrum of in vivo forward mutations, hypermutations, and mutational hotspots in a retroviral shuttle vector after a single replication cycle: substitutions, frameshifts, and hypermutations.Proceedings of the National Academy of Sciences, 1990
- Retroviral recombination during reverse transcription.Proceedings of the National Academy of Sciences, 1990
- Ordered Interstrand and Intrastrand DNA Transfer During Reverse TranscriptionScience, 1988
- DNA polymerase insertion fidelity. Gel assay for site-specific kinetics.Journal of Biological Chemistry, 1987
- High Efficiency DNA-Mediated Transformation of Primate CellsScience, 1983
- Structure, Replication, and Recombination of Retrovirus Genomes: Some Unifying HypothesesJournal of General Virology, 1979