Micro-enzyme immunoassay of vasoactive intestinal polypeptide(VIP)-like immunoreactive substance in bovine milk.
- 1 January 1990
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 38 (4) , 960-962
- https://doi.org/10.1248/cpb.38.960
Abstract
A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11-28) of porcine VIP conjugated with .beta.-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl .beta.-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was 0.1 fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100 pmol/l, which was more than eightfold higher than that in normal milk.This publication has 15 references indexed in Scilit:
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