Decrease in effects of muscimol on the dorsal root after repeated administration of diazepam in rats.

Abstract
In in situ experiments on spinal rats transected at the C1 level and on non-spinal rats, muscimol (3 mg/kg, i.v.) depolarized the primary afferent fibers; diazepam (3 mg/kg, i.v.) potentiated this effect. When diazepam was given for 14 days, the effect of muscimol was significantly reduced in non-spinal, but not in spinal rats. Diazepam probably interacts with GABA-ergic mechanisms more closely in supraspinal regions of the rat.