Membrane potential modulates photocycling rates of bacterial rhodopsins
- 1 August 1988
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 27 (16) , 5843-5848
- https://doi.org/10.1021/bi00416a004
Abstract
Effects of membrane potential on photochemical reactions of three retinal-containing chromoproteins in Halobacterium halobium, sensory rhodopsin I (sR-I), bacteriorhodopsin, and halorhodopsin, are described. Each of the three exhibits a decreased rate of thermal decay of its principal intermediate when photoactivted in an artificially energized compared to a deenergized membrane. The similar response of the three pigments suggests a voltage-dependent conformational change common to their respective photocycles. Spectral and kinetic properties of the sR-I photochemical reaction cycle were measured in phototactic H. halobium cells, and differences from in vitro photocycle kinetics were attributable to the electrical membrane potential present in vivo. In vivo sR-I photocycling rates were reproduced in envelope vesicle preparations in the presence of a valinomycin-induced potassium diffusion potential.This publication has 14 references indexed in Scilit:
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