In Vitro Cytotoxicity of Mouse Macrophages Activated by Coculture with Syngeneic Sarcoma Cells

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Abstract
Normal resident peritoneal macrophages from C3D2(C3H/Tif × DBA/2) F1mice were activated in vitro by culturing with semisyngcneic tumour celts. The tumour cells originated from a methylcholanthrene‐induced sarcoma (MCIM) growing in vivo in ascites form. Macrophagc‐mediated cytotoxicity was evaluated after 5 days of in vitro culture, using five different target cells. Semisyngencic (L 929), allogeneic (B16 melanoma), and xcnogeneic (HeLa) tumour cell lines and normal allogencic tibroblast cell lines (3T3, 3T6) were tested. The morphology and kinetics of the cytotoxicity reaction were studied by scanning electron microscopy and compared with release of radioactivity from14C‐thymidine‐labelled target cells. The activated macrophages were able to kill the semisyngeneic, allogeneic, and xenogeneic tumour cell lines tested under conditions that did not affect normal fibroblasis. The requirement for T cells during activation of the macrophages was also tested. The cyioioxicity decreased markedly when T cells were removed from the macrophage cultures before activation or when macrophages from nude mice were used in the experiments.