Rapid determination of anti-enzyme titer in serum using wholeStaphylococcus aureus cells
- 1 July 1982
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 7 (4) , 307-313
- https://doi.org/10.1007/bf02798308
Abstract
Heat-killed, formalin-fixedStaphyloccous aureus (Cowan) cells are shown to be an excellent immunoadsorbent for quickly and accurately measuring the titer of antibodies directed against enzymes. The simple procedure is illustrated by following the course of immunization of a rabbit against mouse uridine 5’t -monophosphate synthase. The antibody titer of a serum sample can be determined in roughly 1 h plus the time to perform the assays of enzyme (antigen) activity.Keywords
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