The N-terminal amino acid residues of gelatin. 1. Intact gelatins

Publisher Website
Google Scholar
Abstract
The N-terminal residues of a number of gelatins were determined using Sanger''s fluoro-dinitrobenzene technique. Glycine was found to be the pre-dominant end group. Serine, threonine, alanine, aspartic acid and glutamic acid residues also occurred. The number-average molecular weight of these samples was calculated assuming one N-terminal residue in each molecule. Average values of 60,000 were in agreement with those obtained by osmotic-pressure methods and support the view of a non-branching peptide chain. The method was used to determine the lysine residues of gelatin. Generally, over 90% of the e -amino groups of lysine were available to the reagent.