Fluorometric determination of the total DNA content of different yeast species using 4′, 6-diamidino-2-phenyl-indol-dihydrochloride
- 1 December 1985
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 31 (12) , 1164-1166
- https://doi.org/10.1139/m85-219
Abstract
A highly sensitive method has been developed to measure quantitatively the DNA content of yeast cells. The reaction between the fluorescent dye 4′, 6-diamidino-2-phenyl-indol-dihydrochloride and DNA in a crude lysate from protoplasts can be measured without prior extraction of the DNA. Interferences by the high protein and RNA content of yeast cells, which often limit the accuracy of common methods employed in the determination of DNA content, were eliminated by suitable enzymatic pretreatment of the cell lysate.This publication has 4 references indexed in Scilit:
- Protoplast fusion in the yeast, Schwanniomyces alluviusMolecular Genetics and Genomics, 1982
- Assay for nanogram quantities of DNA in cellular homogenatesAnalytical Biochemistry, 1979
- Simple and rapid fluorimetric method for DNA microassayAnalytical Biochemistry, 1977
- The nucleic acids in a polyploid series of SaccharomycesArchives of Biochemistry and Biophysics, 1952