On-Line Micellar Electrokinetic Chromatography−Electrospray Ionization Mass Spectrometry Using Anodically Migrating Micelles
- 1 May 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 69 (10) , 1820-1826
- https://doi.org/10.1021/ac961202+
Abstract
On-line micellar electrokinetic chromatography (MEKC)−electrospray ionization mass spectrometry (ESIMS) is demonstrated for the analysis of chlorotriazine herbicides and barbiturates. In this study, the micellar velocity is directly manipulated by the adjustment of electroosmosis rather than the electrophoretic velocity of the micelle. The electroosmotic flow is adjusted against the electrophoretic velocity of the micelle by changing the solution pH in MEKC. The elimination of MEKC surfactant introduction into ESIMS is achieved with an anodically migrating micelle, moving away from the electrospray interface. The effects of moving surfactant boundary in the MEKC capillary on separation efficiency and resolution of triazine herbicides and barbiturates are investigated. The mass detection of herbicides and barbiturates sequentially eluted from the MEKC capillary is acquired using the positive and negative electrospray modes, respectively.Keywords
This publication has 27 references indexed in Scilit:
- Analysis of Tamoxifen and Its Metabolites by On-Line Capillary Electrophoresis−Electrospray Ionization Mass Spectrometry Employing Nonaqueous Media Containing SurfactantsAnalytical Chemistry, 1996
- Mechanistic Studies of Partial-Filling Micellar Electrokinetic ChromatographyAnalytical Chemistry, 1996
- On‐line combination of micellar electrokinetic chromatography and mass spectrometry using an electrospray–chemical ionization interfaceRapid Communications in Mass Spectrometry, 1995
- Electroosmotic flow control and monitoring with an applied radial voltage for capillary zone electrophoresisAnalytical Chemistry, 1992
- Factors affecting direct control of electroosmosis using an external electric field in capillary electrophoresisAnalytical Chemistry, 1991
- Electrokinetic chromatography: An interface between electrophoresis and chromatographyTrAC Trends in Analytical Chemistry, 1989
- Capillary zone electrophoretic separation of peptides and proteins using low pH buffers in modified silica capillariesAnalytical Chemistry, 1988
- Factors controlling electroosmotic flow in open‐tubular capillaries in electrokinetic chromatographyJournal of High Resolution Chromatography, 1986
- Capillary zone electrophoresis: Effect of physical parameters on separation efficiency and quantitationJournal of High Resolution Chromatography, 1985
- Spectrophotometrically determined ionisation constants of 13 alkylamino-s-triazines and the relationships of molecular structure and basicitySpectrochimica Acta Part A: Molecular Spectroscopy, 1967