The influence of tetradecanoyl-phorbol-acetate (TPA) on histamine release from isolated rat mast cells
- 1 June 1988
- journal article
- research article
- Published by Springer Nature in Inflammation Research
- Vol. 24 (1-2) , 40-48
- https://doi.org/10.1007/bf01968078
Abstract
A detailed investigation of the influence of tetradecanoyl-phorbol-acetate (TPA) on isolated rat mast cells was undertaken in order to explore the possible involvement of protein kinase C in histamine release. TPA alone could induce histamine release in a medium without calcium, whereas 1 mM CaCl2 suppressed the release. TPA in combination with a low concentration of the ionophore A23187 induced a considerable histamine release. Preincubation with TPA followed by incubation with the ionophore induced a similar release at low concentrations of TPA (≦2.5 nM) whereas the response was reduced at higher concentrations of TPA. The inhibition after preincubation with TPA was almost at a maximum within 2 min and was due to a decreased rate of release. TPA could also increase antigen-induced histamine release. After preincubation the potency of low concentrations of TPA increased, whereas higher concentrations (50 nM) became inhibitory. The effects of preincubation were almost fully expressed after 2 min and were not due to altered kinetics of the release. The interaction of oleoylacetylglycerol (OAG) with the ionophore A23187 and with antigen resembled that of TPA, but OAG was considerably less potent. Preincubation with TPA was inhibitory to the histamine release induced by compound 48/80, particularly in the absence of calcium. The release induced by TPA and the ionophore or antigen was calcium-dependent and energy-requiring, and the effects of TPA persisted after washing the cells before exposure to antigen or the ionophore. Preincubation with the protein kinase C inhibitor isoquinolinesulfonyl-methylpiperazine (H-7) slightly enhanced the histamine release induced by the combination of TPA and the ionophore. The suppression exerted by preincubation with TPA on ionophore- and antigen-induced release was counteracted by H-7. The results indicate that only the inhibitory effects of protein kinase C are affected by H-7. Although not conclusive, the results are compatible with an involvement of protein kinase C in both the enhancing and the inhibitory effects of TPA on mast cell histamine release.This publication has 48 references indexed in Scilit:
- Characteristics of the effect of phorbol ester on rat mast cells: Interaction with anti-IgEInflammation Research, 1986
- Two roles for guanine nucleotides in the stimulus-secretion sequence of neutrophilsNature, 1986
- REVIEWBiological Chemistry Hoppe-Seyler, 1986
- The phorbol ester receptor: a phospholipid-regulated protein kinaseBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1985
- Synergism between lysophosphatidylserine and the phorbol ester tetradecanoylphorbolacetate in rat mast cellsLife Sciences, 1985
- Stimulation and inhibition of secretion by phorbol myristate acetate in different cell typesBiochemical and Biophysical Research Communications, 1985
- Protein kinase C as a bidirectional regulator of cell functionTrends in Pharmacological Sciences, 1985
- Phorbol myristate acetate stimulates formation of phosphatidyl inositol 4-phosphate and phosphatidyl inositol 4,5-bisphosphate in human plateletsBiochemical and Biophysical Research Communications, 1984
- 12‐O‐Tetradecanoylphorbol 13‐acetate stimulates inositol lipid phosphorylation in intact human plateletsFEBS Letters, 1984
- Synergistic action of protein kinase C and calcium for histamine release from rat peritoneal mast cellsBiochemical and Biophysical Research Communications, 1984