Butler Modified Wright Stain for Demonstration ofCampylobacter pylori

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Abstract
Campylobacter pylori, a gram negative spiral bacteria, has been found to be the causative infectious agent in many gastric and peptic ulcers and it is necessary to demonstrate C. pylori in tissue sections for a prompt diagnosis. Many laboratories use a modified Steiner method to demonstrate the organism. Although this silver stain works very well, silver precipitation can become a problem with fixatives other than formalin. Precipitation renders the microorganisms difficult to identify, especially when they are present in scant amounts. The Butler Modified Wright stain, which utilizes Weigert iron hematoxylin, Wright stain solution, and safranin, adequately stains C. pylori without masking the bacteria. It is time and cost efficient and provides satisfactory results with formalin fixed tissue, as well as with Hollande and Bouin fixed tissue.
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