Iron-Dependent Transcription of the frpB Gene of Helicobacter pylori Is Controlled by the Fur Repressor Protein
Open Access
- 15 August 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 183 (16) , 4932-4937
- https://doi.org/10.1128/jb.183.16.4932-4937.2001
Abstract
We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake ( frpB and ceuE ) and oxygen radical detoxification ( katA and tsaA ). DNase I footprint analysis showed that Fur binds specifically to a high-affinity site overlapping the P frpB promoter and to low-affinity sites located upstream from promoters within both the frpB-katA and ceuE-tsaA intergenic regions. Construction of an isogenic fur mutant indicated that Fur regulates transcription from the P frpB promoter in response to iron. In contrast, no effect by either Fur or iron was observed for the other promoters.Keywords
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