Multiple components in horse-radish peroxidase
- 1 April 1954
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 56 (4) , 631-639
- https://doi.org/10.1042/bj0560631
Abstract
The peroxidases of horse-radish juice and the latex serum of Ficus macrophylla were examined by filter-paper electrophoresis. Horse-radish contains possibly 5 components with peroxidase activity; Ficus macrophylla, one. There is great seasonal variation in the relative amounts of the peroxidase components present in horse-radish juice; one of the 2 major components is absent in the late spring. A major component is always present in horse-radish juice which corresponds to the peroxidase II of Theorell; other material probably corresponding to his peroxidase I is also present. Two components were discovered which are negatively charged at pH 5.0 and cannot be equated with either peroxidase I or n. One of these has a positive peroxidatic activity against benzidine and little or none against quaiacol; all the components show some differences in reactivity towards various substrates.Keywords
This publication has 13 references indexed in Scilit:
- Factors Influencing the Paper Chromatography of Some Enzymes of Aspergillus Oryzae and Related EnzymesAustralian Journal of Biological Sciences, 1953
- Paper Electrophoresis of Polyglutamyl PeptideNature, 1953
- ‘Double Fronting’ in Paper Chromatography of ProteinsNature, 1952
- Multiple Nature of the Enzymes of Aspergillus Oryzæ and of Horse-Radish: Horse-Radish PeroxidaseNature, 1952
- Multiple Nature of the Enzymes of Aspergillus Oryzæ and of Horse-Radish: Enzymes of Aspergillus oryzaeNature, 1952
- Pitfalls in Partition ChromatographyNature, 1952
- Movement of Proteins on Filter PaperNature, 1951
- Movement of Proteins on Filter PaperNature, 1951
- Purification of horse-radish peroxidase and comparison of its properties with those of catalase and methaemoglobinBiochemical Journal, 1951
- [Electrophoresis of proteins on filter paper].1950