A Fruiting Body Tissue Method for Efficient Agrobacterium -Mediated Transformation of Agaricus bisporus
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Open Access
- 1 October 2000
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (10) , 4510-4513
- https://doi.org/10.1128/aem.66.10.4510-4513.2000
Abstract
We describe a modified Agrobacterium -mediated method for the efficient transformation of Agaricus bisporus . Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.Keywords
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