The nucleotide sequence of bovine MHC class IIDQB andDRB genes
- 1 January 1990
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 31 (1) , 37-44
- https://doi.org/10.1007/bf00702487
Abstract
The nucleotide sequences of most of the exons and parts of the introns of twoBoLA-DQB genes and twoBoLA-DRB genes have been determined. The structure of these genes is very similar to that of human major histocompatibility complex (MHC) class II genes. The twoDQB genes probably represent true alleles. Based on the exons sequenced, bothDQB genes and one of theDRB genes seem to be functional. The otherDRB gene is a pseudogene; stopcodons are found in the exons encoding the second and transmembrane domain and, furthermore, a 2 base pair (bp) deletion has occured in the leader exon which places the initiation start codon out of frame. Also in this pseudogene, an almost perfect inverted repeat of 200 bp is found flanking the exon encoding the first domain, which might have been the result of a duplication/inversion event. The sequences presented in this paper do not contain any repetitions. Therefore, DNA fragments containing these sequences can be used as homologous bovine probes in restriction fragment length polymorphism (RFLP) analysis to study disease association in cattle.Keywords
This publication has 28 references indexed in Scilit:
- The nucleotide sequence of the bovine MHC class II alpha genes:DRA, DQA, andDYAImmunogenetics, 1990
- Genetic polymorphism of horse serum protein 3 (SP3)Animal Genetics, 1989
- The polymerase chain reactionTrends in Genetics, 1989
- Identification of genetic variation in the bovine major histocompatibility complex DRß‐like genes using sequenced bovine genomic probesAnimal Genetics, 1988
- Linkage relationships in the bovine MHC region. High recombination frequency between class II subregionsImmunogenetics, 1988
- Genomic hybridization of bovine class II major histocompatibility genes: 1. Extensive polymorphism of DQα and DQβ genesAnimal Genetics, 1986
- Genomic hybridization of bovine class II major histocompatibility genes: 2. Polymorphism of DR genes and linkage disequilibrium in the DQ‐DR regionAnimal Genetics, 1986
- Transcription termination and 3′ processing: the end is in site!Cell, 1985
- GENETICS OF HLA DISEASE ASSOCIATIONAnnual Review of Genetics, 1981
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976