Possibility of age regulation of the natriuretic peptide C-receptor in human platelets

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Abstract
Natriuretic peptide binding sites on platelets have been hypothesized to act as clearance receptors; however, there is no clear definition of the function of this receptor. The aim of the study was: 1) to characterize natriuretic peptide receptors in human platelets by original competition study; 2) to evaluate a possible age modulation of these binding sites, since a delayed clearance of ANP in the elderly has been observed. The binding of 125I-ANP to intact platelets was completely inhibited by h-ANP, h-BNP, h-CNP and c-ANP, the selective ligand of the clearance receptor. IC50 values were 0.089±0.029, 0.703±0.104 and 1.19±0.13, 3.84±0.04 nmol/l, mean±SE, respectively (pmax 13.6±0.92 fmol/109 platelets (mean±SE), (no.=46, mean age 41.7±2.1 years). Bmax was significantly reduced in older subjects (no.=25, mean age 53.2±1.5 years) with respect to the younger group (no.=21, mean age 28.0±0.87 years): 11.4±1.1 vs 16.1±1.4 fmol/109 cells, p=0.0096, respectively; moreover, a significant inverse relationship between Bmax and the subject’s age was observed. This observation suggests a possible reduction of the natriuretic peptide clearance with aging, associated to a significant increase of plasma levels of natriuretic peptides.

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