Intragenic mutations of the p16 INK4 , p15 INK4B and p18 genes in primary non‐small‐cell lung cancers
- 15 March 1996
- journal article
- Published by Wiley in International Journal of Cancer
- Vol. 65 (6) , 734-739
- https://doi.org/10.1002/(sici)1097-0215(19960315)65:6<734::aid-ijc4>3.0.co;2-#
Abstract
The p15(INK4B), p16(INK4) and p18 genes are members of the gene family coding for inhibitors of cyclin-dependent kinases 4 and 6. p15(INK4B) and p16(INK4) are located at 9p21, a chromosomal region frequently deleted in many human neoplasms. To examine the role of these 3 genes in lung carcinogenesis, somatic mutations within the genes were analyzed by single-strand conformation polymorphism and DNA sequencing in 71 non-small-cell lung cancer (NSCLC) samples. Six somatic mutations in the p16(INK4) gene and 3 cases with a polymorphic allele were observed. Loss of heterozygosity in the p18 gene was found in 1 sample. We did not find any intragenic mutations in the p15(INK4B) or p18 genes. We conclude that p16(INK4) mutations play a role in the formation of some NSCLCs, whereas the involvement of p15(INK4B) and p18 is uncommon.Keywords
This publication has 11 references indexed in Scilit:
- Growth suppression by p18, a p16INK4/MTS1- and p14INK4B/MTS2-related CDK6 inhibitor, correlates with wild-type pRb function.Genes & Development, 1994
- Mutations and altered expression of p16INK4 in human cancer.Proceedings of the National Academy of Sciences, 1994
- Frequent somatic mutations and homozygous deletions of the p16 (MTS1) gene in pancreatic adenocarcinomaNature Genetics, 1994
- Germline p16 mutations in familial melanomaNature Genetics, 1994
- pl5INK4B is a potentia| effector of TGF-β-induced cell cycle arrestNature, 1994
- Somatic Mutations of the MTS (Multiple Tumor Suppressor) 1/CDK41 (Cyclin-Dependent Kinase-4 Inhibitor) Gene in Human Primary Non-small Cell Lung CarcinomasBiochemical and Biophysical Research Communications, 1994
- Rates of p16 ( MTS1 ) Mutations in Primary Tumors with 9p LossScience, 1994
- A Cell Cycle Regulator Potentially Involved in Genesis of Many Tumor TypesScience, 1994
- Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancersNature, 1994
- A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4Nature, 1993