VACUOLES OF FUNGAL PROTOPLASTS

Publisher Website
Google Scholar
Abstract
Details are given of the conditions which allow vacuolar structures of Fusarium culmorum to be released after osmotic bursting of mycelial protoplasts. Steps in the release of vacuoles are shown. The composition of the suspending medium affects the formation, size, and number of the vacuoles seen in the protoplasts. Fusion of vacuoles in regenerating protoplasts has been observed, indicating a self-sealing property of the tonoplast. Preparations of vacuolar structures, rather free from cytoplasmic debris, have been obtained.