The Interaction of Alba, a Conserved Archaeal Chromatin Protein, with Sir2 and Its Regulation by Acetylation
- 5 April 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5565) , 148-151
- https://doi.org/10.1126/science.1070506
Abstract
The conserved Sir2 family of proteins has protein deacetylase activity that is dependent on NAD (the oxidized form of nicotinamide adenine dinucleotide). Although histones are one likely target for the enzymatic activity of eukaryotic Sir2 proteins, little is known about the substrates and roles of prokaryotic Sir2 homologs. We reveal that an archaeal Sir2 homolog interacts specifically with the major archaeal chromatin protein, Alba, and that Alba exists in acetylated and nonacetylated forms. Furthermore, we show that Sir2 can deacetylate Alba and mediate transcriptional repression in a reconstituted in vitro transcription system. These data provide a paradigm for how Sir2 family proteins influence transcription and suggest that modulation of chromatin structure by acetylation arose before the divergence of the archaeal and eukaryotic lineages.Keywords
This publication has 12 references indexed in Scilit:
- Identification of a Conserved Archaeal RNA Polymerase Subunit Contacted by the Basal Transcription Factor TFBPublished by Elsevier ,2001
- Common Themes in Mechanisms of Gene SilencingMolecular Cell, 2001
- The complete genome of the crenarchaeon Sulfolobus solfataricus P2Proceedings of the National Academy of Sciences, 2001
- Crystal Structure of a SIR2 Homolog–NAD ComplexCell, 2001
- Mechanism and regulation of transcription in archaeaCurrent Opinion in Microbiology, 2001
- Coupling of histone deacetylation to NAD breakdown by the yeast silencing protein Sir2: Evidence for acetyl transfer from substrate to an NAD breakdown productProceedings of the National Academy of Sciences, 2000
- Phylogenetic Classification of Prokaryotic and Eukaryotic Sir2-like ProteinsBiochemical and Biophysical Research Communications, 2000
- An Enzymatic Activity in the Yeast Sir2 Protein that Is Essential for Gene SilencingCell, 1999
- Transcriptional Regulation of an Archaeal Operon In Vivo and In VitroMolecular Cell, 1999
- Identification of the gene encoding archeal‐specific DNA‐binding proteins of the Sac10b familyMolecular Microbiology, 1999