Spectrophotometric Method for the Estimation of 6-Aminopenicillanic Acid
Open Access
- 1 February 1973
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 3 (2) , 194-197
- https://doi.org/10.1128/aac.3.2.194
Abstract
A simple, rapid, and sensitive method is described whereby 6-aminopenicillanic acid can be spectrophotometrically determined in the presence of penicillins and their degradation products without prior separation. d -(+)-Glucosamine is used as reagent. The effect of such parameters as p H, temperature, and time of heating on the formation of the chromophore is described. The recommended range is from 25 to 250 μg of 6-aminopenicillanic acid.Keywords
This publication has 3 references indexed in Scilit: