Stereochemistry of 1-(4′-hydroxyphenyl)ethanol produced by hydroxylation of 4-ethylphenol by p-cresol methylhydroxylase
- 1 December 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 224 (2) , 617-621
- https://doi.org/10.1042/bj2240617
Abstract
Enzymic hydroxylation of 4-ethylphenol by (a) Pseudomonas putida and (b) highly purified p-cresol methylhydroxylase gave optically active 1-(4′-hydroxyphenyl)-ethanol. The products were transformed into the phenolic methyl ethers and shown to contain 69.5% and 65.6%, respectively, of the (S)-(-)-isomer. The stereochemistry of the reaction is discussed in terms of three distinct steps occurring at the active site of the enzyme.This publication has 9 references indexed in Scilit:
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