Lack of evidence for skeletal abnormalities in offspring of mice exposed to ethylnitrosourea.

Abstract
Using morphometrical methods, variation was investigated in the skeletons of more than 400 offspring of C57BL/6J and DBA/2J male mice that had received either a 250 mg/kg dose of the mutagen ethylnitrosourea or a solvent control. Sperm involved in the matings developed from cells in the spermatogonial stage at the time the animals were injected. Although variants were detected, differences in frequencies of gross abnormalities and minor variations in shape between treated and control groups were almost all nonsignificant. There were also no major differences in measures of variability within the groups of offspring from either the treated males or the control group. Additional examination of the skeleton for changes in the frequency of a series of nonmetrical variants also provided no evidence of differences between the treated and control groups that could be attributed to induced mutations. These results conflict with previous findings that ethylnitrosourea is a potent inducer of dominant skeletal mutations.

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