β-naphthoflavone induction of CYP1A in brain of juvenile lake trout(Salvelinus namaycush Walbaum)
Open Access
- 1 April 2004
- journal article
- research article
- Published by The Company of Biologists in Journal of Experimental Biology
- Vol. 207 (9) , 1533-1542
- https://doi.org/10.1242/jeb.00919
Abstract
SUMMARY Many environmental pollutants induce expression of the cytochrome P450 (CYP) 1A subfamily of genes. We integrated cellular and molecular biological techniques to examine the effects of β-naphthoflavone (BNF) exposure in lake trout brain CYP1A distribution and dynamics. Over a 32-day time-course, real time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) results showed that CYP1A mRNA induction in response to BNF exposure occurred rapidly and continued to rise in the BNF-treated lake trout after 4 h, with a peak at or after 2 days. Messenger RNA levels fell after 4 days, and this trend continued after 16 days of exposure. In situ hybridization indicated that CYP1A mRNA was universally elevated in the brain of BNF-exposed fish and was mainly expressed in the endothelia and occasionally in the glial cells. CYP1A immunoreactivity was induced in the olfactory bulb and valvula cerebelli of BNF-treated fish. Other brain areas showed constitutive CYP1A immunoreactivity in both control and BNF-treated fish. Some BNF-treated fish contained multifocal hemorrhages in the brain tissue, and these fish had overall depressed CYP1A immunoreactivity in the brain. The relationship between transcriptional and translational effects of BNF exposure in the brain of juvenile lake trout is discussed.Keywords
This publication has 47 references indexed in Scilit:
- 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicity in the Zebrafish Embryo: Local Circulation Failure in the Dorsal Midbrain Is Associated with Increased ApoptosisToxicological Sciences, 2002
- Tissue-Specific Expression of AHR2, ARNT2, and CYP1A in Zebrafish Embryos and Larvae: Effects of Developmental Stage and 2,3,7,8-Tetrachlorodibenzo-p-dioxin ExposureToxicological Sciences, 2002
- Immunochemical approaches to studies of CYP1A localization and induction by xenobiotics in fishPublished by Springer Nature ,1998
- Effects of PCB on xenobiotic biotransformation enzyme activities in the liver and 21-hydroxylation in the head kidney of juvenile rainbow troutAquatic Toxicology, 1997
- Distribution and induction of cytochrome P450 1A1 in the rainbow trout brainFish Physiology and Biochemistry, 1994
- 2 Unique CYP1 Genes Are Expressed in Response to 3-Methylcholanthrene Treatment in Rainbow TroutArchives of Biochemistry and Biophysics, 1994
- Pituitary as a target organ for toxic effects of P4501A1 inducing chemicalsMolecular and Cellular Endocrinology, 1993
- The cytochrome P-450 system in fish, aquatic toxicology and environmental monitoringAquatic Toxicology, 1992
- Glucocorticoid-mediated potentiation of P450 induction in primary culture of rainbow trout hepatocytesBiochemical Pharmacology, 1992
- Axonal Transport of Cadmium in the Olfactory Nerve of the PikeBasic & Clinical Pharmacology & Toxicology, 1991