Real-Time PCR to Determine Transgene Copy Number and to Quantitate the Biolocalization of Adoptively Transferred Cells from EGFP-Transgenic Mice
Open Access
- 1 September 2008
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 45 (3) , 247-258
- https://doi.org/10.2144/000112913
Abstract
Quantitative real-time PCR (qPCR) is a sensitive technique for the detection and quantitation of specific DNA sequences. Here we describe a Taqman qPCR assay for quantification of tissue-localized, adoptively transferred enhanced...Keywords
Funding Information
- the EGFP-transgenic mouse strains
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