The Effect of Medium Constituents on Growth and Diosgenin Production byDioscorea deltoideaCells Grown in Batch Cultures
- 1 February 1982
- journal article
- research article
- Published by Georg Thieme Verlag KG in Planta Medica
- Vol. 44 (02) , 111-115
- https://doi.org/10.1055/s-2007-971414
Abstract
The effect of nitrogen and carbon sources on growth and diosgenin production was studied with Dioscorea deltoidea cells which were grown under defined conditions in a chemostat, and transferred to batch cultures. Both ammonium and nitrate nitrogen were required for growth and steroid production; diosgenin production was maximal at higher amounts of nitrogen. The nature and concentration of the carbon source (sugar) had marked effects on diosgenin production. A maximal amount of diosgenin (3.8% of dry wt.) was produced on sucrose (15 g/liter) after 21 days of growth in a batch culture.Keywords
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