Protoplast regeneration from normal and bromodeoxyuridine-resistant sycamore callus
Open Access
- 1 November 1974
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 16 (2) , 445-463
- https://doi.org/10.1242/jcs.16.2.445
Abstract
Protoplasts have been prepared from normal and mutant lines of sycamore callus, maize root and tobacco leaf. Fusion was rare in sodium nitrate solutions. A mutant tissue culture was selected for by its resistance to 10 µg/ml bromodeoxyuridine. The mutant is sensitive to the Hypoxanthine-Aminopterin-Thymidine (HAT) selective medium but has normal thymidine transport and thymidine kinase activity.Keywords
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